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REBECCA TROYACK RIBEIRO DA SILVA SEGUNDO replied to the topic "PBM in patients "

Publication: Red and infrared laser exposure upregulates NF-kappaB/p65 in triple-negative HCC1937 breast cancer cells.

Very good your presentation Rebecca! Since infrared laser exposure activates NF-κB and upregulates IL-6 and IL-8 in BRCA1-mutant HCC-1937 cells, could these photobiomodulation parameters potentially interfere with cancer therapy or promote tumor progression these patients?

Question

REBECCA TROYACK RIBEIRO DA SILVA SEGUNDO and 1 other person replied to the topic "Cell line"

Publication: Red and infrared laser exposure upregulates NF-kappaB/p65 in triple-negative HCC1937 breast cancer cells.

First of all, Rebecca, congratulations on a truly fantastic presentation. I was wondering if you intend to expand your research to other cellular models, specifically those that are not BRCA1-mutant, to determine if the effects of the red and infrared lasers are similar?"

Question

KASSIANE DA SILVA RIBEIRO replied to the topic "NTZ treatment"

Publication: GENETIC POLYMORPHISMS IN THE VDR GENE AND THEIR INFLUENCE ON NATALIZUMAB RESPONSE IN MULTIPLE SCLEROSIS PATIENTS

Amazing presentation Kassiane! In your project, all your patients were using the NTZ treatment? Were there a negative control of patients who didn't take this drug?

Question

KASSIANE DA SILVA RIBEIRO and 1 other person replied to the topic "Next steps"

Publication: GENETIC POLYMORPHISMS IN THE VDR GENE AND THEIR INFLUENCE ON NATALIZUMAB RESPONSE IN MULTIPLE SCLEROSIS PATIENTS

Your work is very interesting! What are the next steps you have planned?

Question

KASSIANE DA SILVA RIBEIRO and 1 other person replied to the topic "SNPs associados à falha terapêutica"

Publication: GENETIC POLYMORPHISMS IN THE VDR GENE AND THEIR INFLUENCE ON NATALIZUMAB RESPONSE IN MULTIPLE SCLEROSIS PATIENTS

Parabéns pelo trabalho, ficou muito bom! Sei que não é o seu foco, mas fiquei curiosa sobre, você ou o seu grupo pensam em testar alguma combinação do natalizumabe com algum outro fármaco para os casos das pessoas com genótipos associados à falha terapêutica descrita?

Question

Gabrielle da Silva Brugger and 1 other person replied to the topic " A2 β-casein "

Publication: Gene Regulation of β-Casein and Its Application to Health

Hello Gabrielle, good morning. Congratulations on the presentation. How can genetic selection for the A2 β-casein variant influence consumer health while also affecting the productive efficiency of dairy herds?

Question

Mariana Berquó Carneiro Ferreira replied to the topic "Methylation "

Publication: Epigenetic regulation of the HES1 gene and aspects of neurodevelopment in premature twins

Hello! I would like to congratulate on your fantastic job! I was wondering if methylation of the HES1 gene help predict how a preterm baby will develop?"

Question

Gabrielle da Silva Brugger replied to the topic "Growing number of people with milk protein allergies"

Publication: Gene Regulation of β-Casein and Its Application to Health

Hello, I was very curious about your presentation. I wanted to ask if, based on this literature review, it's possible to infer that the growing number of people, especially babies, with milk protein allergies could be related to this polymorphisms derived from β-casein? Recently, I started noticing this A2 milk on the supermarket shelves, so does that mean it's better than A1?

Other

Ana Clara Cavallo Dobao replied to the topic "Gene choice"

Publication: Evaluation of the Effects of Photobiomodulation with Low-Power Red Laser and Ultraviolet A LED on Cell Survival and Antioxidant Markers in MCF-7 Breast Cancer Cells

Good evening, Ana Clara. Congratulations on your work; the quality of life of cancer patients is extremely important. Ana, do you plan to evaluate other genes? If so, which ones do you consider essential for studying photobiomodulation in tumor cells?

Other

Ana Clara Cavallo Dobao replied to the topic "PBM and Proliferation Gene Regulation in MCF-7 Cells"

Publication: Evaluation of the Effects of Photobiomodulation with Low-Power Red Laser and Ultraviolet A LED on Cell Survival and Antioxidant Markers in MCF-7 Breast Cancer Cells

Great presentation Ana Clara! Considering that PBM with red laser and UVA-LED did not modulate survival or antioxidant genes but increased MKI67 expression, how do you interpret the specificity of PBM in regulating proliferation-related genes in MCF-7 cells?

Question

Ana Clara Cavallo Dobao and 1 other person replied to the topic "Effects of red laser "

Publication: Evaluation of the effects of low-power lasers on gene expression of stem phenotype markers and breast cancer spheroids viability

Hello, Luiza, good afternoon! Congratulations on the great presentation and incredible work! I have a question: I would like to know if the investigation of the effects of these radiations is aimed at cancer treatment or if it is to develop safer protocols for cancer patients to use for the therapeutic purposes mentioned in the video? I would also like to know if you plan to investigate other cellular events, besides cell proliferation. Thank you!

Question

Mariana Berquó Carneiro Ferreira replied to the topic "Why did you choose to study twins?"

Publication: Epigenetic regulation of the HES1 gene and aspects of neurodevelopment in premature twins

First, I'd like to say that I really enjoyed your work and was intrigued by it. I wondered: why did you choose to study twins? I thought perhaps it was to have a baseline, since each pregnancy is unique, but each baby is different, so why study twins?

Question

Luiza de Lima Silva replied to the topic "Follow-up on NANOG expression and functional effects"

Publication: Evaluation of the effects of low-power lasers on gene expression of stem phenotype markers and breast cancer spheroids viability

Congratulations on your presentation! Do you plan to repeat the experiment on NANOG expression, and if the levels remain elevated, do you intend to investigate the effects of this increase, considering that this technique is used for the treatment of other diseases?

Question

Luiza de Lima Silva replied to the topic "Nanog expression "

Publication: Evaluation of the effects of low-power lasers on gene expression of stem phenotype markers and breast cancer spheroids viability

Hi, Luiza Fantastic presentation and well discussed! I was wondering if you and your group are willing to investigate why only NANOG expression was affected by red laser?

Question

Maria Eduarda Barbosa Mourão replied to the topic "Next steps"

Publication: THE BASAL SUBTYPE HAS HIGHER mRNA LEVEL EXPRESSION OF THE TNF-alpha PATHWAY THAN THE LUMINAL SUBTYPE IN BREAST CANCER SAMPLES.

Your work is very interesting! What are the next steps you have planned? Are you planning to carry out further experiments to strengthen these findings?”

Question

Paulo Henrique dos Santos Bento replied to the topic "Testing with the cell line from the referenced study"

Publication: The role of the NS5A protein of the hepatitis C virus (HCV) in cellular signaling pathways associated with hepatocellular carcinoma in the presence of oxidative stress

Congratulations on your presentation! Do you plan to perform tests using the cell line from the article you mentioned, since your results showed the opposite of what was reported in that study?

1 person favorite the paper "GENETIC POLYMORPHISMS IN THE VDR GENE AND THEIR INFLUENCE ON NATALIZUMAB RESPONSE IN MULTIPLE SCLEROSIS PATIENTS"

Question

Paulo Henrique dos Santos Bento replied to the topic "Impact of NS5A on oxidative stress and oncogenic pathways"

Publication: The role of the NS5A protein of the hepatitis C virus (HCV) in cellular signaling pathways associated with hepatocellular carcinoma in the presence of oxidative stress

Congratulations for your work, it's very interesting! I'm currently studying the NS5 protein in gliomas, so I found your study extremely relevant and engaging. I have two questions about your work: First, how might the upregulation of GRB2 by NS5A contribute to the establishment of a pro-oncogenic microenvironment in hepatocytes? And given that NS5A expression protected cells from H2O2-induced loss of viability, what additional experiments would you propose to clarify whether this protective effect is mediated primarily through oxidative stress modulation or through alterations in survival signaling pathways?

Question

Marcelle Abreu da Silva replied to the topic "Methylation"

Publication: Methylation profile analysis of LINE1, HES1, and SLC6A4 in premature preschool children

Congratulations on an excellent presentation, Anna Beatriz My question is regarding your methodology. I understand that DNA was collected during the first months of life and then again in the preschool years. Could you please explain the rationale for choosing the seven-year follow-up time point? Also, do you plan to collect more DNA methylation data from these children in the future to understand the long-term dynamics of this phenomenon?

Question

Marcelle Abreu da Silva replied to the topic "Epigenetics"

Publication: DNA Methylation As An Epigenetic Link Between Childhood Adversities And Inflammatory Bowel Diseases

Congratulations on an excellent presentation, Geovanna. My question is regarding your methylation analysis. You focused on the promoter region, but since methylation can also occur in other areas, such as gene bodies, do you plan to expand your study to investigate those regions as well? Additionally, what are the next steps for your project?

Question

Marcelle Abreu da Silva replied to the topic "Epigenetics"

Publication: Epigenetic regulation of the HES1 gene and aspects of neurodevelopment in premature twins

Congratulations on a very insightful project, Mariana. My question is: Did you consider adverse events throughout each twin's development, such as infections or length of NICU stay? These are factors that could potentially influence DNA methylation and/or neurodevelopment. Additionally, do you plan to expand the work to analyze other epigenetic mechanisms, such as histone modification?

Question

Marcelle Abreu da Silva replied to the topic "Cell line"

Publication: The role of the NS5A protein of the hepatitis C virus (HCV) in cellular signaling pathways associated with hepatocellular carcinoma in the presence of oxidative stress

Congratulations on an excellent presentation, Paulo Henrique. I have a question regarding your methodology. You used the HEK293FT cell line, which are kidney cells, ritgh? Considering that chronic HCV infection is characterized by a persistent inflammatory process in the liver, as noted by the WHO, could you explain the rationale for choosing kidney cells over hepatocytes for example? Do you anticipate that the results might differ significantly had you used a different cell model?

Question

Letícia Santiago da Silva Ferreira replied to the topic "Questions"

Publication: Decoding the epigenetic response to early stress: SLC6A4 methylation trajectories in preterm newborns

Thanks for your amazing talk and results! I have some questions, as follows: 1) Given the temporal changes in CpG methylation you observed, have you assessed whether these epigenetic modifications also happen at the transcriptional or protein level of SLC6A4 to establish functional consequences beyond methylation signatures? In addition, did you explore whether the CpG-specific methylation differences cluster within known transcription factor binding sites or regulatory motifs of the SLC6A4 promoter, and could these site-specific changes differentially impact serotonin transporter regulation? 2) How do you distinguished methylation changes due to prematurity itself from those potentially driven by external perinatal stressors (e.g., oxygen therapy, corticosteroids, NICU handling, maternal stress), and have you controlled for these in your analyses? 3) From a translational perspective, do you envision that SLC6A4 methylation profiling at birth could serve as a biomarker for identifying preterm infants at higher risk of later neurodevelopmental disorders, and what are the ethical implications of early-life epigenetic risk stratification in neonatology? Thanks and congrats again!

Question

Letícia Santiago da Silva Ferreira replied to the topic "Socioeconomical differences between groups"

Publication: Decoding the epigenetic response to early stress: SLC6A4 methylation trajectories in preterm newborns

First I would like to compliment you on your great work and presentation! Really great. Secondly, I wanted to know if you normalized your data based on the differences in family income (FI) to see if, in the same stratification of FI these methylation differences persisted between the groups. We know that socioeconomical differences impact in what the mother eats and their level of stress during pregnancy, which could have an effect in DNA methylation regardless of birth term.

1 person favorite the paper "Methylation profile analysis of LINE1, HES1, and SLC6A4 in premature preschool children"

1 person favorite the paper "DNA Methylation As An Epigenetic Link Between Childhood Adversities And Inflammatory Bowel Diseases"

1 person favorite the paper "Epigenetic regulation of the HES1 gene and aspects of neurodevelopment in premature twins"

1 person favorite the paper "The role of the NS5A protein of the hepatitis C virus (HCV) in cellular signaling pathways associated with hepatocellular carcinoma in the presence of oxidative stress"

1 person favorite the paper "Red and infrared laser exposure upregulates NF-kappaB/p65 in triple-negative HCC1937 breast cancer cells."

1 person favorite the paper "TWIST1 IS A KEY REGULATOR OF IL‑17RA IN BREAST CANCER"

2 persons favorite the paper "Decoding the epigenetic response to early stress: SLC6A4 methylation trajectories in preterm newborns"

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