Fast determination of ascorbic and isoascorbic acid by capillary electrophoresis

Vol. 1, 2019. - 116618
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Abstract

This work aimed to validate an analytical method to determine ascorbic and isoascorbic acids in fresh and commercial orange juices samples. Figure 1 illustrates the separation of analytes under optimized analytical conditions. Samples were diluted four-fold with 1000 mg/L EDTA and centrifuged. LOD and LOQ were estimated as 3 and 10 mg/L, respectively. Evaluation of linearity (10 to 200 mg/L) showed good coefficients of correlation (r > 0.99). Recovery was assessed at 50, 100 and 200 mg/L of ascorbic and isoascorbic acids ranged from 100-107 and 105-106%, respectively. Precision (n=3) varied from 0.62 to 2.3% and 1.6 to 3.9% for ascorbic and isoascorbic acid, respectively. Validated method was used to analyze freshly squeezed orange juices and diverse commercial samples. Concentration of ascorbic acid ranged from 112 to 567 mg/L in fresh orange juice and from <LOQ to 304 mg/L in commercial samples. None samples had isoascorbic acid concentration above LOQ.

Institutions
  • 1 Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP), Universidade de São Paulo (USP)
Keywords
ascorbic acid
orange juice
capillary electrophoresis