Antioxidant Activity of BRS Violet Grape Juice Residue from Extraction Process
The process that grapes undergo for juice manufacturing, in both industrial and small-scale agriculture, generates an abundancy of agroindustrial residue, which can be used as base for the development of new nutrient’s alternatives, as well as the development of new food products [1]. Grapes pomace resulted from wine and grape juice production contains a variety of bioactive compounds that could provide important natural antioxidants properties that are able to prevent diseases caused by free radical [2] [3] [4] [5]. Therefore, this research’s main objective was to evaluate the antioxidant activity in pomace from grape juice obtained using steam juicers from the BRS Violeta grape, given that this cultivar has high polyphenols amounts [6]. The pomace obtained from the grape juice production’s surplus utilizing steam juicers was lyophilized and the sample’s extract preparation occurred in the following manner: 2 g of lyophilized sample was added in a 25 mL of 50% ethanol at 50 °C for 45 minutes at 150 rpm. The antioxidant activity was evaluated through three methodologies (ABTS, DPPH e FRAP) and the content of phenolic compounds was determined by Folin-Ciocalteau method. The pomace’s antioxidant activity determined by DPPH, ABTS and FRAP methods was respectively 429 ± 0.0 mg TEAC g-1, 3765.31 ± 0.02 mmol TEAC g-1 and 1165.0 ± 0.0 mmol Fe2+ g-1. The amount of phenolic compounds found was of 11758 ± 39 mg GAE 100 g-1. Results such as this are expressive when compared to those in the literature [5]; for instance, analysis using Pinot Noir grapes pomace obtained higher DPPH value and lower ABTS value and phenolic compounds, which may have occurred as a consequence of the extraction method or due to the different grape variety. In addition, it is possible to affirm that pomace from grape BRS Violeta juice production may come to add value to this species production, as it, regarding residue from extraction process, exhibits high antioxidant activity. As a result, the pomace may come to be considered a residue with potential usage.