PIPELINE PARA ANÁLISE DA EXPRESSÃO DIFERENCIAL DE GENES E DESCOBERTA DE SNPS EM ÉXONS UTILIZANDO DADOS DE RNA-SEQ

RNA-Seq, a tool for gene expression analysis, generates a great amount of data which requires bioinformatics knowledge from users. The objective of this study was to develop algorithms that simplify gene expression and SNP discovery from RNA-Seq data evaluation, using a Linux based and a more user-friendly web-based platform, Galaxy. The initial steps include the quality control and trimming of the reads. For gene expression, reads were aligned to a reference genome and the annotation data recovered. After that, reads were calculated and the differential expression information obtained. For SNP discovery, the same archives were aligned to a reference genome and the process of calling was performed. The developed algorithms were able to reduce complexity of bioinformatics tools for gene expression and SNP discovery from RNA-Seq data.