The western diet and sedentary lifestyle are among the biggest contributors to obesity development. Studies have shown that adipose tissue dysfunction may be one of the major determinants of metabolic deficiencies in obese individuals, especially visceral adipose tissue, such as the mesentery. Therefore, the present study aim to evaluate the high fat diet (HFD) and the diet with substitution of 10% of flaxseed (FS) influence after 3 days of consumption. Animals C57BL/6J, 4 weeks old, were used. We carried out measurements such as food intake, fasting glucose, body weight gain, metabolic and physiological parameters and lipidomics of adipose tissue. After 3 days of treatment, it was observed that the HFD animals presented statistical difference in the total final weight, as well as in the food intake and in the adipose tissues final weight. On the other hand, animals FS showed lower caloric intake when compared to the HFD group. The omega-3 fatty acids from flaxseed (FS) reached this tissue in abundance, differently from another diets (CTL and HFD). The short period of high-fat diet consumption can acutely changes the total body weight, food intake and visceral adiposity, despite to it the glucose homeostasis does not change. In the other hand, flaxseed oil was able to gently protect against metabolic disturbances. More analyses should be done to clarify the pro- and antiinflammatory flaxseed properties and its incorporation into adipose tissues.