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Effectiveness of four methods of teeth sterilization and their influence on the proteolytic activity of bovine dentin

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It was evaluated the effectiveness of four methods used for teeth sterilization and their influence on proteolytic activity of bovine dentin. The methods investigated were: gamma radiation, ultraviolet light, ethylene oxide, and autoclaving. Except for ultraviolet light, all methods were considered effective for teeth sterilization, however, in situ Zymography showed that these methods increased the proteolytic activity of bovine dentin.