Soluble guanylyl cyclase (sGC) acts as the principal intracellular receptor for nitric oxide (NO), facilitating the generation of cyclic GMP. Soluble guanylyl cyclase is a heterodimer complex consisting of two subunits, α and β, each of which contains three domains, namely one domain N-terminal regulator, a region of dimerization and a C-terminal domain that is responsible for substrate recognition and activity catalytic of GCs, which is responsible for the conversion of GTP to cGMP.