Chitosomes are structures formed by electrostatic complexation of chitosan with phospholipid bilayers of liposome that aim to improve efficiency of liposomes in terms of stability and mucoadhesive properties as a delivery system of bioactive compounds. This study focused on analysis of operational parameters, particles characterization and study of chitosan–phospholipids interactions in order to obtain positively charged particle with nanometric size and low polidispersity index chitosomes.