Anais do Simpósio Nacional de Bioprocessos
XXII National Bioprocesses Symposium (SINAFERM) XIII Enzymatic Hydrolysis of Biomass Symposium (SHEB)

Allergy to the cow milk is a major cause of food allergies, which can be overcome by proteolysis of its proteins. For this purpose, immobilized enzymes are more advantageous because easier reaction control and biocatalyst reusability. In this work, the protease Novo-Pro D™ was immobilized on agarose support activated with glyoxyl groups and chitosan support activated with glutaraldehyde, yielding immobilization yields up to 93.5%, however, yielding recovered activities of 91.9% and 34.7%, respectively. The best biocatalyst was around 7 times more stable than the soluble dialyzed enzyme at 50 °C and pH 8.0.