Potential Hydrolytic Activity Study of Moringa and Sunflower Seed Lipases

Moringa oleifera LAM (moriga) and H. anuus L. (sunflower) are plants resistant to dryness and great commercial importance, due to edible oil production. However, little information is available concerning sunflower and moringa seed lipase. Thus, the aim of this work is to accomplish lipase extraction obtained from germinated seeds from moringa and sunflower and verifying the hydrolytic potential of these lipases. The hydrolysis of olive oil of moringa lipase presented the best result in 1 hour of extraction, liberating 3513.47 µmol.g-1 of fatty acids in 10 minutes of reaction. For sunflower seed lipase, the best result was obtained in 3 hours of extraction, totaling 3.004,8 µmol.g-1 of fatty acid in 10 min of reaction. For moringa and sunflower seeds lipases the optimum pH was 4.0 showing hydrolytic activity of 755.42 ±10.06 U.g-1 and 841 ±11.07 U.g-1, respectively. In both lipases the optimum temperature was 40 ° C, with activity of 1463.00 ± 9.12 U.g-1 for moringa lipase and 979.02 ±15.91 U.g-1 for sunflower lipase.