OPTIMIZATION OF LIPOPEPTIDE PRODUCTION AND ANTIADESIVE ACTIVITY

The biosurfactants are viable alternatives to chemical surfactants for their better performance, in addition to being biodegradable, low in toxicity and can be derived from renewable agricultural residues. They are stable to variations in temperature, pH and salinity, present low critical micellar concentration (CMC) and high surface activity. Is a molecule of great interest to industry for variety of applications. However, its cost of production is still higher than the synthetic one due to the low yield and high cost of the downstream stage. From the fractional factorial analyzes, some additional experiments were carried out, which allowed an increase in yield of 8.5 times, from 65.04 to 556.56 mg/L, presenting a biofilm antiadhesive activity of approximately 95%. By means of a central composite design (DCCR), it was observed that the chosen concentrations of L-arginine ranges only affect the production and concentration of 1g/L yielded the highest concentration of lipopeptide. The cost of producing 1g of lipopeptide when using the alternative medium with soybean meal extract was about 16 times lower when compared to the modified culture medium. This bioprocess could be a lower cost way to produce lipopeptide with the interest of the industry.