Low cost complex culture medium for syngas fermentation by Clostridium carboxidivorans

Three different complex culture media were tested for Clostridium carboxidivorans syngas fermentation. Tryptone, Yeast extract and Glucose medium (TYG) and Tryptone, Peptone, Yeast extract, Glucose and L-arginine medium (TPYGarg) were compared to Brain Heart Infusion medium (BHI) usually used for Clostridium cultures. TYG and TPYGarg are reduced in cost and in number of components in relation to BHI. TPYGarg medium led to an increase of 97% on biomass concentration, 66% on ethanol production, and 77% on butanol concentration compared to BHI medium, achieving 1.36 g d. w. cells/L, 1.11 g/L of ethanol, and 0.71 g/L of butanol. Besides, this medium (TPYGarg) is 70% cheaper than BHI.