Characterization of amino-functionalized rice husk silica and its use as support to immobilize Thermomyces lanuginosus lipase.

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Abstract

This study aimed to prepare an ion-exchange support (Amino–SiO2) via functionalization of rice husk silica (RHS) with (3-aminopropyl)triethoxysilane (3–APTES). Several methods were used to characterize the introduction of aminopropyl groups on the RHS surface, such as Attenuated total reflectance – Fourier transform infrared (ATR–FTIR), Thermogravimetric (TGA) and differential thermal (DTA), and transmission electron microscopy (TEM) analyses. Functionalized support was used to immobilize Thermomyces lanuginosus lipase (TLL) via ionic interactions.

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Institutions
  • 1 Universidade Federal de Alfenas
  • 2 Instituto de Química (UNIFAL) / Universidade Federal de Alfenas
  • 3 Universidade Federal de Minas Gerais
Track
  • 6. Enzymatic Processes
Keywords
Lipase
Rice husk silica
Enzime immobilization
Ion-exchange support