Biosurfactant Production by Bacillus invictae in submerged fermentation using alternative medium

The biosurfactants are molecules produced by bacteria, fungi and yeasts grown in different carbon sources as result of their metabolism. Are amphiphilic compounds produced on living surfaces, or excreted extracellularly moieties that confer the ability to accumulate between fluid phases, reducing surface tension. The aim of the present study was to investigate the biotechnology potential of Bacillus invictae UCP 1617 for biosurfactant production. The production was carried out using the medium of residue: corn oil, corn steep liquor and pineapple juice obtained from the bark. The Erlenmeyer’s 250 ml we are incubated at 30 °C, at 150 rpm during 72 hours. And the liquid metabolic of free cells was used to evaluate reduction of surface tension, emulsification index, toxicity test. The results showed the bacteria B. invictae water reduction of surface tension of 69.97 ± 0.12 to 30.73 ± 0.12 mN.m-1. The emulsification activity rates were observed with motor oil, diesel, gasoline, kerosene, soybean oil, corn and sunflower oils. The toxicity test used Artemia salina showed lower toxicity. The biotechnological results showed higher potential of B. invictea UCP 1617 to biosurfactant production and suggest the use of pineapple peel waste for tensio-active agent production as a low-cost medium.