Hydrogen is a high commodity chemical product also considered as an energy vector which is produced by different routes, including fermentation process. After production, hydrogen should be separated from other gases in order to achieve the required purity. A promising separation alternative is the use of palladium membranes, which present an infinite selective to hydrogen. In this study, a composite palladium/alumina membrane was evaluated for hydrogen permeation. The palladium film of 920 nm was electroless deposited on an asymmetric alumina hollow fiber. The hydrogen permeability through the membrane was of 1.62x10-3 mol m-2 s-1 kPa-1, at 450 °C, with hydrogen/nitrogen selectivity of 30. These results indicate the need of a thicker palladium layer in order to increase membrane selectivity and also the investigation of the roughness of the outer surface of the hollow fiber in order to avoid the formation of pinholes.