The enzymatic catalysis for biodiesel production using low-cost, high-acid, lower-grade waste oils has attracted much attention recently due to economic issues, renewability and sustainable benefits. Performance of a commercial low cost lipase (Eversa® Transform, Novozymes) was investigated in the esterification reactions for fatty acid methyl esters (FAME) production. In order to reduce the costs of industrial enzymatic biodiesel production, enzyme was used in its soluble, free form, as well as low cost substrate the non-edible waste cooking oil was employed towards FAME production. FAME yields as high as 96% were achieved using 2.5 wt% water, 1.5 methanol equivalent and 1 wt% Eversa®Transfom enzyme content in 16 h reaction. After studied laboratory, scale-up of biodiesel production increased 6 times de reaction of FAME production. The enzyme biodiesel process described in this study appears to be a promising alternative to the traditional biodiesel process and can contribute to make the enzymatic biodiesel feasible.