Nanofibers have a high surface area in relation to the volume and can be used in the protection of unstable compounds and easy degradation. Various types of natural and synthetic polymers are employed in the manufacture of nanofibers, such as poly (L-lactic acid) (PLLA), which exhibits characteristics such as biodegradability, toxicity and biocompatibility with human cells and tissues. In biomedical applications, nanofibers can act as a support for bioactive compounds. Phycocyanin is a natural compound found in cyanobacteria and has antioxidant, anti-inflammatory and antitumor properties. The objective of the work was to produce PLLA nanofibers added with 1% phycocyanin for possible application as scaffold. Using this concentration, nanofibers of 722 ± 147 nm in diameter were obtained. The diameter of the capillary and the distance from the capillary to the collector were fixed at 0,45 mm and 150 mm, respectively. Through fluorescence microscopy and phase contrast analysis, the incorporation of phycocyanin to the nanofibers was confirmed. The porosity of the nanofibers was 83.7 ± 0.6%. Thus the development of biodegradable polymer nanofibers associated with bioactive compounds for use as scaffold can bring significant contributions in the field of tissue engineering.