Bisphenol A (BPA), an endocrine disruptor compound, is released into the environment and barely degraded in wastewater treatment plants. The aim of this work is to investigate the ability of laccases from Pleurotus ostreatus and Pleurotus pumonarius to degrade BPA. BPA at 100 mg/L was completely removed in a 1 hour reaction by the laccases of both fungi present at a concentration of 8 U/L. The acute BPA toxicity decreased from 85% to 25% using the P. ostreatus laccase. Using the P. pulmonarius laccase, however, an increase of toxicity was observed, indicating the generation of compounds that are more toxic than BPA. The results of this study suggest a feasible method for the complete removal of BPA from polluted environments using P. ostreatus laccase.