Horseradish peroxidase (HRP) was immobilized on Amberlite IRA 67, weak base resin impregnated with copper ions in different concentrations (0.03 and 0.3 M) . The effect of the loading enzyme was evaluated. Also, for immobilized enzyme, thermal stability and pH stability were investigated. Immobilization efficiency decreased with a loading 5 mg HRP/g support to impregnated support with 0.3 and 0.03 M copper ions. The optimal temperature and pH of the immobilized enzyme in Amberlite impregnated with 0.3 M and 0.03 M copper ions were 30 °C and 7.0. HRP immobilized on Amberlite impregnated with 0.3 M showed to be more thermally stable.