Large volumes of waste are generated during the manufacture of the peach-palm (Bactris gasipaes Kunt.). The use of this waste as substrate to enzyme production by microorganisms is a viable alternative. In present work we evaluated the best culture conditions to increase the xylanase production by Trichoderma stromaticum AM7 in solid state fermentation using peach-palm waste. There was an increase of 160% in xylanase production after performing optimization techniques using the Box-Behnken statistical design. The study of fermentation optimization in peach-palm waste indicated that maximum activity occurred when the nitrogen concentration was 1.0 % after 6 days of cultivation at 32 ° C, reaching 1440 U/gds. The use of tropical fungal microbiota from the degradation of peach-palm waste proved to be a viable alternative for the generation of xylanase enzyme and the results of this study are discussed in the context of obtaining low cost fermentation methods with satisfactory yields of xylanase activity with potential for biotechnological application.