Nanofibers are one of the newest and most promising nanomaterials for a number of applications. It is possible to obtain nanofibers from various polymers by the electrospinning technique. Biodegradable and biocompatible polymers like poly (D,L lactic acid) (PDLLA) are the best candidates for the preparation of nanofibers. One possible nanofibers application is the development of scaffolds in tissue engineering and active packaging. Phycocyanin is a natural, water-soluble and light sensitive compound found in cyanobacteria and red algae, and has antioxidant, anti-inflammatory and antitumor properties. The aim of this work was to develop nanofibers of 25% PDLLA added with 0.5% and 1% of phycocyanin with potential application in tissue and food engineering. With addition of 0.5% and 1% of the bioactive, were produced nanofibers of 921±180 nm and 997±191 nm in diameter, respectively, compound under 150 μL h-1 and 24.3 kV. The incorporation of bioactive compounds into nanofibers may confer thermal stability to thermally unstable compounds and act as support for application in tissue and food engineering fields.