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The proposal of this work was based on the possibility of immobilization of Lipase NS 40116 in polyurethane foam (PUF) support from green sources. The reaction of castor oil and glycerol, using enzymatic glycerolysis produced mono- and diacylglycerol, which were used as biopolyol for obtaining a support for immobilization of the enzyme using the entrapment technique. The characterization of immobilized enzyme was performed by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and apparent density. A hydrolytic enzyme activity of 6.62 U/g was observed in the immobilized lipase.