In the last decades, the demand for high quality products, coupled with the intense search for the use of "green" technologies, has attracted interest in different sectors. This situation reflects directly in the industrial processes, since the quality of the final product is associated with the technology used in its production. Thus, it is required the development of new processes based on the required minimum use of energy and solvents without environmental restrictions. In this context, technologies based on the use of supercritical fluids appear to offer solutions to these demands. The objective of this work was to investigate the extraction of Lupinus albescens roots using supercritical CO2 as a solvent, as well as the chemical characterization of the extracts. The results were compared with the Soxhlet conventional technique using n-hexane as the solvent. The highest yield obtained using supercritical extraction was 0.34% at 60 °C and 250 bar, higher than the conventional method with n-hexane, which presented 0.20%. The chemical compound that presented the largest area for both extractions was stigmasterol. It is concluded that the extraction of the root of L. albescens using the supercritical CO2 method proved to be an effective technique.