In this work, a supermacroporous polyacrylamide cryogel was synthesized and activated with tris hydroxymethyl aminomethane (Tris) as affinity ligand aiming the separation of lysozyme from chicken egg white (CEW). The effects of dilution rate of CEW and flow velocity were analyzed and it was observed that flow velocity did not affect purification parameters such as extraction efficiency and yield. This result suggest that the support might be useful for purification of lysozyme from CEW.