The objective of the present work was to characterize the lignocellulosic material obtained after the process of delignification of sugarcane bagasse using hydrogen peroxide (H2O2). The experimental conditions varied from 25-45°C for the temperature and from 1.5-7.5% (v/v) for the H2O2 concentration. The sample obtained after pretreatment of the bagasse was characterized from Raman spectroscopy, where it was possible to observe the signal emission in the characteristic scattering of the presence of aromatic rings in the structure, indicating that there are lignin fractions. The experimental condition suggested as the most adequate for the process was that using 1.5% (v/v) H2O2 at 25°C.