Surfactants are substances widely used in various industrial sectors, however, they are produced synthetically from petroleum. Within this context, research aimed to sustainable production of surfactants has gained worldwide importance. Biosurfactant is an amphiphilic molecule produced by microorganisms, presenting advantages such as: biodegradability, less toxicity, activity in higher
temperature range and pH, besides being able to be produced from industrial residues and byproducts, such as sugarcane bagasse (SCB), and hence, reducing production costs. Solid state fermentation is a promising process for the production of the biosurfactant because it does not produce foam, found concern in process of submerged fermentation. Thus, the objective of this work was to define ethodological conditions, through statistical design of experiments (24), for the
production of biosurfactant by the yeast Aureobasidium pullulans LB83 from SCB, studying the variables: biomass fiber size, medium volume, cell concentration and volume of distilled water used for extraction. According to the statistical analysis, bagasse size and the volume of initial medium presented significant positive and negative effects in the response answer biosurfactant concentration, respectively. The presented SSF proposal was shown as sustainable and promisor approach.