Aspergillus is a fungal genus, which is widely used in the development of biotechnological processes and commonly applied in the production of valuable industrial enzymes. The objective of this work includes bioprospecting enzyme production from Aspergillus tamarii URM 4635 through Solid-state fermentation and its characterization by electrophoresis. The microorganism was maintained and incubated with the Czapek culture medium for sporulation for 72 hours at 36°C. In the vial containing the fermented FT substrate was added deionized water, containing 0.1% (w / v) Tween 80, placed on the orbital shaker at 160 rpm at room temperature (28°C) for 1 hour. The content were pressed to obtain Enzymatic Extract (crude extract- EB). The supernatant (EB) was analyzed for different enzymatic activities as follows: protease, collagenase, pectinase, endoglucanase and fructosyltransferase. The higher enzymatic activities detected was for collagenase 210.6 (U/mL). Aspergillus tamarii URM4634 has demonstrated a has a pronounced potential for several enzymes of industrial interest, being able to be used for several applications such as cosmetics, leather, pharmaceutical, food, as well as animal nutrition.