Polyhydroxyalkanoates (PHA) are an option to diminish the impact caused by inadequate disposal of synthetic plastics. In this study, the evaluation and characterization of PHA production by the strains C. necator IPT 026 and 027, and B.cepacia IPT 119 and 400, using soybean as carbon source, was carried out. All microorganisms tested were able to utilize the substrate offered to synthetize PHA, which showed the characteristic bands of polyesters functional groups in the infrared spectra (FTIR). The highest production performance was achieved by C. necator IPT 027, exhibiting 0.84 ± 0.07 g L-1 of polymer in the synthesis medium.