The objective of the study was to determined potential biogas and methane production from biomass of microalga Heterochlorella luteoviridis. The biomass was cultivated in bench-scale photobioreactors. Biomass fermentation was conducted in 250 mL batch tests at 37.0 ± 2.0 °C. The biogas production was (530 LN Biogas kg SV-1) and methane yield of 75 %. This result suggest that biomass was readily utilized by anaerobic microorganisms indicating high biodegradability of biomass compounds.