High Spatiotemporal precision in Live Sample Imaging: Advancements with Lattice Light-Sheet Microscopy

The inherent four-dimensional nature of biological processes — comprising time and space — necessitates high temporal resolution and volumetric imaging techniques for an improved understanding of underlying mechanisms.
The lattice light-sheet microscope (LLSM) uses an ultra-thin sheet of light uniquely illuminates the plane of the imaging, greatly reducing the effect of photobleaching and phototoxicity. This, combined with fast volumetric acquisition within sub-second intervals attained with diffraction-limited resolution, results in a noninvasive four-dimensional (time, space) visualization fluorescence optical visualization technique suited to image over a broad scale, from single molecules to organelles in cells in isolation and within tissues.
This talk will illustrate our use of LLSM and the updated version with adaptative optics (AO-LLSM) suited to image tissues to characterize a range of biological events. These examples will include the uncovering of stable octameric templates of nuclear porins seed the very fast post-mitotic assembly of nuclear pore complexes, discovery of the Notch synapse between cells and its inherent dynamics to mediate Notch signaling, and single virion tracking as they enter cells during early steps of infection.