EXPRESSION AND PURIFICATION OF A HEAT SHOCK PROTEIN SMHSP FROM AEDES AEGYPTI

The research involves the characterization of a SmHSPs protein from Aedes aegypti, which are named and characterized for having monomeric structures with lower molecular masses, ranging from 12 to 42 kDa. They act in preventing the formation of aggregates by binding to exposed hydrophobic regions due to an increase in temperature, for example. Based on this, this work aims to express, purify, and characterize a SmHSP from Aedes aegypti using biophysical and biochemical techniques, in order to understand the mechanisms involved in maintaining the mosquito's proteostasis, as it is the vector responsible for transmitting emerging viruses such as Zika (ZIKV), Dengue (DENV), Chikungunya (CHIKV), and Yellow Fever (YFV).