2-Phenylethanol is a well know alcohol that is characterized by its rose-like odor, being used as component in fragrance, cosmetic, and food industries1. It occurs widely in nature, mainly in essential oils extracted from rose, jasmine, carnation, and hyacinths2. However, their low concentration makes the recovery complex and expensive1,2. 2-Phenylethanol was also described as showing antimicrobial and antityrosinase activities, which aggregates more attention to its production3. 2-Phenylnitroethane, used in this work as substrate for 2-phenylethanol, is the main component of Aniba canelilla essential oil, corresponding to approximately 80% of the total amount. Then, it was used as substrate without any previous purification. To produce 2-phenylethanol through biotransformation of 2-phenylnitroethane, four different fungi were applied: Aspergillus flavus and Rigidoporus lineatus (isolated from copper mining residue from Mina do Sossego, Canaã dos Carajás, Pará, Brazil), Lasiodiplodia caatinguensis (phytopathogenic fungus from Citrus sinensis), and Colletotrichum sp. (phytopathogenic fungus from Euterpe oleracea). The fungi were cultivated in Erlenmeyer flasks (250 mL) containing 100 mL of malt extract solution (2%) for seven days under orbital agitation (132 rpm) at 30 ºC. Then, 50 mg of A. canelilla essential oil were diluted in 100 µL of dimethylsulfoxide (DMSO) and added to each fungi cultivation. Each experiment had its control by fungi cultivation without the essential oil addition. Every day (1 - 7 days), aliquots (2 mL) were removed from each experiment and extracted using ethyl acetate (2 mL) and analyzed by GC-MS in order to determine the percentage of 2-phenylnitroethane biotransformation in 2-phenylethanol. After 7 days, it was observed that the two fungi (A. flavus, and L. caatinguensis) performed the complete biotransformation of 2-phenylnitroethane in 2-phenylethanol, with special attention to L. caatinguensis that achieved the complete biotransformation after two days of reaction. The other two fungi (R. lineatus, and Colletotrichum sp.) partially performed the biotransformation, but Colletotrichum sp. also produced a not-identified compound, which will the object of future studies. Therefore, it was possible to assign that A. flavus and L. caatinguensis fungi are very promisors in 2-phenylethanol production using 2-phenylnitroethane as substrate and can be the object of study in the next steps of this work, that includes the improvement of the reactional conditions and the production of 2-phenylethanol using other substrates such as hydrolyzed lignin and cinnamyl alcohol.