Bergenin is a gallic acid C-glycoside derivative that presents in vitro and in vivo different pharmacological and biological activities such as, anti-inflammatory, antiasthma, hepatoprotective, among others. This compound can be found in different plant families, but it is common especially in Fabaceae. Peltophorum dubium (Spreng.) Taubert (Fabaceae) is a tree popularly known as “canisfítola”, “faveira”, “angico amarela” or “farinha-seca” and its root is a source of bergenin. This work describes the preparation of a molecularly imprinted polymer (MIP) employing bergenin as an active center and submits the methanolic extract of the roots of P. dubium to isolate pure bergenin. The MIPs are synthetic material possessing specific sites and it is obtained from scaffold compounds and functional monomers that react furnishing the polymers with recognize the scaffold molecule. In the present study, the MIP was prepared employing methacrilic acid (MAA), bergenin (scaffold molecule), ethylene glycol dimethacrilate (EGDMA) as cross-linking reagent, AIBN as radical initiator and MeOH:toluene (1:1) mixture as the porogenic solvent. Sequentially, the MIP was eluted with methanol, obtaining the leached MIP. Bergenin was extracted from the methanolic extract of the roots of P. dubium, using the conventional SPE procedure employed as stationary phases the leached MIP. The leached and un-leached MIP were analyzed by IR spectroscopy and the determination of bergenin realeased was performed using HPLC/DAD analyzes with validated parameters.