The bioaccessibility, defined as the amount of metal that is converted to soluble forms in the gastrointestinal conditions can be evaluated by sequential analysis with artificial gastric and intestinal juices. The analysis of the soluble fractions is widely used to evaluate bioaccessibility of minerals and trace elements from different food products [1]. In this study, the bioaccessibility of Cd, Cu, Fe and Zn was accessed in basil samples after Cd intoxication. The seeds were germinated in Germitest paper until the roots length ~ 0.5 cm. Afterwards, radicle were grown in hydroponic solution, using 5% v v-1 Hoagland solution. The cuttings were exposed to Cd2+ [as Cd(NO3)2.4H2O] to varying concentrations (0, 1.5 and 3.0 mol L-1). Hoagland solution were replaced each 3 days and after 15 days the plants were transferred to vermiculite pots and kept for another 15 days, to increase the quantity of leaves. In vitro gastrointestinal [2] were applied in fresh and lyophilized samples. After, 1 mL of the digested sample were sonicated in a cup horn ultrasound, adding 100 μL HNO3 and 500 μL H2O2. Final volume was completed to 2 mL. Total concentration was achieved after microwave (MW) acid digestion. Limits of detection and quantification after MW digestion for Cd, Cu, Fe and Zn were 0.07 and 0.23, 0.6 and 1.99, 10.7 and 35.3, 8.5 and 27.9 g L-1, respectively. Total concentrations and bioaccessibility values presented in Table 1 were obtained after quantification by ICP-MS.