In the present work, the synthesis of bis-aldimines as potential soil urease inhibitors has been developed. Ureases are part of a class of enzymes that catalyze the hydrolysis of urea to the formation of ammonia, water and carbon dioxide. Despite the low cost, urea when applied to the soil, suffers loss of nitrogen by volatilization when hydrolyzed. With this limitation, the development of potentials urease inhibitors is fundamental, since the national market does not have this technology. Aldimines, inspired by the reaction between tiocarbohydrazone and aldehydes, may be an alternative as urease inhibitors due to structural similarity with thiourea to bypass large nitrogen losses through the hydrolysis of urea. Were synthesized eight bis-aldimines, which were purified by recrystallization and the yields ranging from 46 to 89%.