Nitric oxide is a free radical, gaseous at room temperature, colorless, highly reactive and one of the main mediators of intra and extracellular processes. Endogenously produced nitric oxide has been studied extensively in the past years owing to its roles in numerous physiological processes, which include neurotransmission, vasodilatation, blood pressure regulation, and angiogenesis. In vivo NO measurements are challenging because such compound is present in low concentrations in the body (sub µM levels) and is highly reactive. Thus, the development of sensitive and selective NO electrochemical sensors is an exciting challenge to be faced. Accordingly, in this work we show our efforts to modify the surface of a Pt microelectrode (r = 25 µm) by platinization for further application of such a platform for the anodic oxidation of NO. The platinization was performed at controlled potential in a H₂PtCl₆ + 0.5 M H₂SO₄ solution for varying deposition times. The electrochemical oxidation of NO occurs at around 0.7 V (vs. Ag/AgCl) for the bare Pt microelectrode, whereas the roughened electrode surface of the platinized Pt microelectrode enables faster electron-transfer kinetics. As a consequence, a much sharper voltammetric wave is noticed with a remarkable current enhancement. This is possibly attributed to the formation of metallic surface sites, which facilitate the electron-transfer step, associated with the significant increase in surface area. The enlarged surface is responsible for the increased capacitive current, as readily observed in the voltammograms recorded with the platinized Pt microelectrode. Notwithstanding, in comparison to bare Pt microelectrode, the platinized one showed enhanced sensitivity for NO detection. The influence of some experimental parameters such as deposition potential, deposition time, and H₂PtCl₆ concentration on the anodic oxidation of NO was systematically investigated.