Phytochemical study of polar phases from Richterago campestris (Asteraceae)

Asteraceae, the second largest family of flowering plants, contains more than 25,000 species and 1700 genera distributed among 43 tribes. Gochnatieae tribe is the sister group of ~96% of the species in Asteraceae and shows 7 genera1. Among them, Richterago comprises 16 species, all endemic to Brazil. Richterago campestris Roque & J.N. Nakaj occurs only in Minas Gerais state2 and no data about its chemical constituents are described in the literature. This work aims to report the first phytochemical study of polar phases from R. campestris. Extracts from aerial parts (68.46 g) of R. compestris were prepared with hexane and subsequently with methanol. Methanol extract was partitioned successively with hexane, dichloromethane, ethyl acetate (EtOAc) and buthanol (BuOH). These groups were analyzed with HPLC- UVDAD. From the EtOAc and BuOH phases were identified through NMR and commercial standards five flavonoids [quercetin-3-O-galactoside (1), quercetin-3-O-glucoside (2), quercetin 3-O-β–(6”-O-E-caffeoyl)- galactoside (3), tiliroside (4), 3’-methoxy-helichrysoside (5)], four chlorogenic acids derivatives [5-O-(E)- caffeoylquinic acid (6), 3,5-di-O-(E)-caffeoylquinic acid (7), 4,5-di-O-(E)-caffeoylquinic acid (8), 3,4-di-O-(E)- caffeoylquinic acid (9)], and the caffeic acid (10). These compounds were reported for the first time in R. campestris.