New steroidal saponins from Solanum paniculatum L. (Solanaceae) leaves.

Solanum paniculatum L. known populary as jurubeba1 is a medicinal plant specie with a bitter taste widespread Tropical America especially in the Brazilian savana region used throughout in Brazil for culinary purposes and in folk medicine to treat gastric dysfunctions, as well as an antifever and a hangovers2. Due to the importance and the use of this plant in Brazil, the aim of our research group was to investigate chemically the 70 % ethanolic extract from the leaves of S. paniculatum L. The 70% ethanolic extract after fractionation using classical chromatographic techniques (liquid-liquid partition, Sephadex LH-20) resulting in a saponin fraction (f26), that after purification by HPLC-RI yielded three new spirostanic saponins 6-O-α-L-ramnopiranosil- (1’’→3’)-β-D-quinovopiranosil-(22S,23R,25S)-3β,6α,23-triidroxi-5α-espirostano (1), 6-O-β-D-xilopiranosil- (1’’→3’)-β-D-quinovopiranosil-(22S,23R,25R)-3β,6α,23-triidroxi-5α-espirostano (2) and 3-O-α-Lramnopiranosil-( 1’’→3’)-β-D-quinovopiranosil-(22S,23S,25R)-3β,6α,23-triidroxi-5α-espirostano (3). The structures of these compounds were elucidated using 1D and 2D NMR experiments along with HRESIMS.