Evaluation of three methodologies to optimization of phycobiliprotein extraction in aqueous fraction of Arthrospira platensis

Introduction: Phycobiliproteins are water pigment-proteins present in the microalga Arthrospira platensis, considering their biological activities it is very important to optimize their recovery 1,2. Objective: to evaluate three methods for phycobiliproteins extraction in order to improve their recovery. Methodology: The three procedures are shown below (Figure 1): The quantification of the phycobiliproteins were carried out according to the equations of Figure 2.: Based on the three procedures performed, it was possible to verify that the freezing / thawing extraction was the most efficient process, or it was the most efficient process to break the cells and exposure the proteins or the less aggressive process to these proteins, or even it might be a combination of these two possibilities.