Chemical composition of bufonids toads from Legal Amazonia

The Bufonidae toad family contains about 52 genera and 607 species. These animals produce and store poison in their parotid glands. The poisons are composed mainly of steroids, biogenic amines and peptides, originated from the secondary metabolism of frogs [1]. These compounds have already presented, in vitro, different pharmacological effects, especially antiproliferative activity [2]. Thus, the objective of this work is to determine the chemical composition of venoms obtained from three different species of bufonids captured in the state of Mato Grosso. Rhinella marina, Rhaebo guttatus and Rhinella schneideri collected in the municipalities of Colniza, Nova Ubiratã and Poroxéu, respectively, were studied. The poisons were obtained by manual compression of the amphibian glands, dried and extracted with methanol. The extracts were analyzed by HPLC-MS-MS [3]. From the bufonids poisons, 31 compounds were identified or tentatively identified by spectrometric mass means. Among them, alkaloids, free bufadienolides and argininyl diacid derivatives of bufadienolides. The argininyl diacids included derivatives from bufalin, marinobufagin, telocinobufagin, hellebrigenin and resibufogenin. The poisons presented high chemical diversity in its constituents. These findings stimulate further work on the chemistry and bioactivity of Legal Amazonia toads.