Chemical characterization of pyriproxifene by chromatography (HPLC-PDA and CG-MS)

The pyriproxyfen has been used in control of drinking water assessment in Brazil for human consumption1, in order to combat the proliferation of larvae Aedes aegypti. However, biological and chemical studies of this larvicide are still recent, so this work is intended to make a screening of insecticide through chromatographic techniques. In HPLC-PDA, 1 mg of the insecticide was used, solubilized Rock in mixture acetonitrile-methanol (80:20), and subjected to the process of clean up in Cartridge Chromabond ® C18ec (previously activated with MeOH), filtered with 0.45 μm membrane, injected into a Shimadzu analytical liquid chromatograph, model LC20A, controller CBM-20, with UV detector, and isocratic method of 0-60 minutes and flow 1.0 ml/min, C18 column (250 x 4.6 mm, particle size 5 μm), Macherey-Nagel brand. In the analysis CG-MS, was injected 10 μL of analyte splitless mode injection, use one capillary column RTX-5MS, model TQ8030 (length 30m, 0, 25mm ID., DF 0.25 μm), coupled to a mass spectrometer. The oven temperature was programmed in: 80 °C (2 min), 20 °C/min at 180 °C and 5 °C/min to 280 °C (3 min), whose gas carrier helium to 1 μL/ min with linear speed 44.5 cm/s and initial pressure 100 kPa. In the analysis of the insecticide by HPLC-PDA in λ of 272 nm (Figure 01), it can be seen the presence of two intense peaks with Retention times (TR) of 3,39min e 4,04min, respectively, corresponding to the wavelengths characteristic of the pyriproxyfen (λ 272 nm) and pyridalyl (λ 208 nm), according to a literature2. The analysis of insecticide by HPLC-PDA and GC-MS allowed the identification the pyriproxyfen and pyridalyl.