Chemical characterization of antidiabetic active ethanolic extract from Jatropha aethiopica (Euphorbiaceae) leaves.

Jatropha aethiopica, popularly known in Cuba as “mata diabetes”, is used traditionally because its antidiabetic properties1. The aim of this work was chemical and biological characterize the ethanolic extract (EE) from Jatropha aethiopica leaves. The fractionation of the EE was performed by sephadex LH-20 yielding eighteen fractions purified by HPLC-PDA preparative. The structure of the compounds were elucidated using 1D and 2D NMR experiments as well as HRESIMS techniques. Three flavonol triglycoside compound were described, being one of them described as a new. The three flavonol triglycosides are quercetin 3-O-- L-rhamnopyranosyl-(12)-[-L-rhamnopyranolsyl-(16)]-β-D-galactopyranoside (1), kaempferol 3-O- - L-rhamnopyranosyl-(12)-[-L-rhamnopyranolsyl-(16)]-β-D-glucopyranoside (2) and, a new kaempferol 3-O--L-rhamnopyranosyl-(14)-[-L-rhamnopyranolsyl-(16)]-β-D-galactopyranoside (3) (Figure 1). The antidiabetic property of the EE from Jatropha aethiopica leaves was previously demonstrated (Figure 2)2.