Bioprospecting anticancer metabolites from Microsphaeropsis arundinis, an endophytic fungi from Paepalanthus planifolius.

Eriocaulaceae family have 1200 species divided into 10 genera. This plants have chemical studies in literature that describe the presence of substances with biological potential, such as planifolin in Paepalanthus planifolius. Despite this, there are few data about the chemistry of the microorganisms associated with these species, such as endophytic fungi. In this study, we study chemically and biologically the endophytic fungus Microsphaeropsis arundinis, isolated from P. planifolius capitula. This endophyte are reported to have caused tissue infections in humans. The endophyte was cultivate in liquid culture media (Potato Dextrose Broth, YM Broth, Malt Extract, Nutrient Broth and Czapek-Dox Broth) and solid culture media (parboiled rice and corn) and the ethyl acetate extracts were biologically evaluated against adenocarcinoma cell lines LM3 (murine breast), LP07 (murine lung) and MCF-7 (human breast) (Table 1). The ethyl acetate extract cultivated in parboiled rice was the most active one against murine breast adenocarcinoma and human breast adenocarcinoma, being select to cultivated on an enlarged scale and fractionated result in the isolation of major substances. The use of spectroscopic and spectrometric techniques result in the identification of aromatic polyketides (1-3), with 1 (1-(2,5-dihydroxyphenyl)-3-hydroxybutanone) described for the first time as a natural product and a macrolide (4) described for the first time in the genus.