PREPARATION AND CHARACTERIZATION OF BIODEGRADABLE FILMS FOR IMMOBILIZATION OF LIPASE

The use of biopolymeric blends as support for enzyme immobilization has gained a prominent position in recent years due to their unique characteristics, including non-toxicity, biodegradability, excellent affinity for proteins, in addition to being renewable and abundant (DE CASTRO et al., 2021). The presence of nanoclay in polymer blends (composites) can act as reinforcing agent, it can improve the quality of the material as support for enzyme immobilization (SIMÕES et al., 2020).
The objective of this work was to produce and characterize biodegradable films containing κ-carrageenan (κ-car), corn starch (CS), Poly(vinyl alcohol) (PVA), glycerol and Sodium montmorillonite (MMT) using the casting method according to BARIZÃO et al. (2020) with modifications, to immobilize lipase from Burkholderia lata LBBIO-BL02 using the sandwich adsorption method.