Biobased Polyelectrolytes as Promoters of Protease Activity

Enzymes have become increasingly important in industry, particularly in the formulation of sustainable detergent products. Their beneficial properties often compete with those of chemical additives such as polymers, requiring protective measures to prevent enzyme denaturation. However, previous studies have indicated that certain polyelectrolytes can enhance enzymatic activity. A deeper understanding of how polyelectrolytes influence protease function could support the development of more effective and sustainable detergent formulations.

This study aims to evaluate the interaction of biobased polyelectrolytes, polyaspartic acid (PASA) and α-poly-L-lysine (α-PLL), with a commercial detergent protease. Protease activities in solution and at the surface were analyzed by fluorescence spectroscopy and quartz crystal microbalance (QCM). Fluorescence correlation spectroscopy (FCS) was employed to study enzyme-polyelectrolyte interactions, while thermal shift assays were used to examine the effects of polyelectrolyte binding on enzyme stability.

The analysis reveals that both polyelectrolytes can modulate protease activity albeit via different mechanisms. These findings highlight their potential for improving enzymatic performance in detergent formulations, contributing to effective and sustainable detergent solutions.