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Characterization of Manihot esculenta tannic leaves and cutting extract and Mimosa caesalpiniifolia peel for additive employment on ruminant diet

Delci D. Nepomuceno ; Michel Cardoso Vieira ; Thainá de Lima Risso ; Leonardo F. Morais ; Roberto Carlos Costa Lelis ; João Carlos C. Almeida
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Condensed tannins (CT) are alternative additives for ruminant feeding employment in order to mitigating methane. Currently, main CT sources are represented by legumes as acacia (Acacia sp) and quebracho (Schinopsis sp). However, CT are described as secondary metabolites from several plant species. Nevertheless, the possibility for extracting Mamilot sculente (cassava) leaves and cuttings has not been known yet. This survey aimed qualifying tannic extract from cassava leaves and cuttings in comparison with that one from Sabi? peel (Mimosa caesalpiniifolia) (arboreal legume). Leaves and cuttings from farmers in Serop?dica municipality, RJ, and Sabi? peel from Dairy Cattle Sector in Universidade Federal Rural do Rio de Janeiro (UFRRJ) in pastures and Animal Nutrition Department were dried and addressed to UFRRJ Forest Products laboratory. Following, samples were milled by hammer mill and by Stiasny methodology tannic extract was obtained. Completely randomized design with three replicates each for M. sculente leaves and cuttings and for Sabi? peel was carried out. Total solid content (extractive), Stiasny index (% CT reacting with formaldehyde on acid medium), CT content and no tannins content (extract impurities) were estimated. Results were subjected to variance analysis and averages compared by Tukey test (P = 0.05). The total extracts content 6.22%; 15.36% and 19.64% and not tannins 1.78%; 14.74% and 18.92% of the sabi? peel, and the leaves and cuttings of cassava, respectively, were increased and differed between the samples (P <0.05). As for the Stiasny index and TC content the sabi? peel showed 71.62 % and 4.44%, these levels which differed (P <0.05) Stiasny index, 3.66% and 4.05% and CT 0.72% and 0.62% of cuttings and leaves, respectively. No significant difference between M. sculente parts for Stiasny index and CT was reported. Low CT content on tannic extracts might be a limiting factor for its extraction employing Stiasny index only with water as solvent, as well.