63236

Malaria-Intestinal Parasite Co-infections: Intensity of Plasmodium vivax Transmission Associated with Specific IgG Responses (PvMSP119) in Individuals from Brazilian Amazon Border Community

Rubens Alex de Oliveira Menezes 1 ; Tamirys Pina Simão ; Margarete do Socorro Mendonça Gomes 2 ; Anapaula Martins Mendes 3 ; Alvaro Augusto Ribeiro D Àlmeida Couto 2 ; Mathieu Nacher 4 ; Martin Johannes Enk 5 ; Maristela Gomes da Cunha 6 ; Ricardo Luiz Dantas Machado
Download
Favorite this paper

Backgrounds: Polyparasitism is a common condition in humans, but its impact on the immune system and clinical diseases is still poorly understood. There are few studies on the prevalence and effect of malaria-intestinal parasite co-infections in the immune response of this interaction. Materials and Methods: We characterized the malaria-intestinal parasite co-infection associated with specific IgG responses (PvMSP119) by evaluating the reactivity and intensity of Plasmodium vivax transmission in446 individuals from a community of the Brazilian Amazon, located in the State of Amapá, Oiapoque municipality, bordering French Guiana, and the samples was collected from 2014 to 2015. The IgG antibodies specific for (MSP-119) were detected in the plasma by means of the enzyme-linked immunosorbent assay (ELISA) and the samples were divided into four groups: group I [Malaria and Parasitosis (n = 118)], group II [Malaria only (n = 34)], group III [Parasitosis only (n = 143)] and group IV [Control (n = 151)]. Results: The reactivity index of the samples ranged from 0.0182 to 18.4364 ± 3.3584 with 53.8% (240/446) presenting His6-MSP119 specific IgG, being these 82.2% (97 / 118) belonging to group I, 76.4% (26/34) to group II, 40.5% (58/143) to group III and 39% (59/151) to group IV.The frequency of antibodies observed was 57.5% (61/106) for protozoa, 57.9% (69/119) for helminths, 69.4% (25/36) for protozoa and helminths and 45.9% (85/185) without infection. Analyzing the frequency in the groups I and III we obtained for group I: 76.9% (30/39) with protozoa infection, 87.5% (49/56) with helminths and 75% (18/24) protozoa and helminths infections; for group III: 45.5% (31/68) with protozoa infections, 31.7% (20/63) with helminths and 58.3% (7/12) with protozoa and helminths infections.Conclusions: Our results confirm the high antigenicity of this protein, especially for group I, presenting a different profile regarding reactivity and intensity of transmission. These data add important information that may help in understanding the immunological aspects involved in the pathogenesis of malaria vivax.