Introduction: Three-dimensional (3D) bioprinting has shown significant advances in the medical field. The fabrication of structures and artificial organs has been essential to the recovery of many individuals affected by illness or accidents, including those who have suffered irreversible bone loss. Objectives: This study aimed to produce scaffolds by 3D bioprinting using bioinks based on bacterial cellulose/alginate for bone repair. Methods: Bioinks were prepared using a base hydrogel (HB) of deconstructed bacterial cellulose (BC) and sodium alginate (ALG) functionalized with active components (graphene anchored with silver and sodium alendronate (GrafBone bioink) or associated with strontium apatite (Apatitebone bioink) to evaluate its ability to induce bone tissue regeneration. The bioinks were characterized regarding their rheological properties. After 3D bioprinting, the scaffolds were characterized by swelling capacity, porosity, mechanical, antimicrobial, cytotoxicity and biocompatibility properties. Results The formulated bioinks were odorless, being a homogeneous mixture with predominant viscoelastic characteristics that change from liquid to solid after 0.2 seconds, demonstrating that they gel quickly. The ApatiteBone bioink showed a more evident thixotropic characteristic, represented by the larger hysteresis area. In the compression tests, the wet reticulated GrafBone bioink showed greater resistance to rupture, requiring a force of 17N, while for the ApatiteBone sample it was only 12N. The bioinks in the test with Artemia at a dose of 1000µg/mL for 48h did not show toxicity or viability of Artemias. Conclusions: The results demonstrated that the synthesis route developed in the study was successful and that the two produced inks are printable, presenting good mechanical resistance and high biocompatibility.