Umari (Poraqueiba sericea) is a widely consumed fruit in the Amazonian region, being known for a high lipid content, and antioxidant and anti-inflammatory properties. However, the polysaccharide profile of umari fruit pulp remains largely unexplored. This study aimed to extract and characterize the complex polysaccharides in the umari fruit pulp, a member of Icacinaceae family. Freeze-dried pulp (60 g) was defatted with chloroform:methanol solution (2:1, v/v), followed by hot water extraction (3x, 1 L each), yielding 3.6 g of water-soluble polysaccharides. After purification using α-amylase and Fehling’s solution, the extracted polysaccharides were fractioned by sequential ultrafiltration through membranes. Two homogeneous fractions, PSF100R and PSF50R, were obtained. PSF100R was primarily composed of arabinose (52%), galactose (19%), glucose (12%), uronic acids (11%), and rhamnose (5%), while PSF50R contained arabinose (69%), galactose (11%), glucose (10%), uronic acids (5%), and rhamnose (3%). 2D HSQC NMR analysis revealed major chemical shifts which could be assigned to the anomeric carbons of of α-L-Araf and β-D-Galpsubunits, along minor shifts associated to anomeric carbons of α-D-GalAp and α-L-Rhap subunits These findings suggest that these fractions are arabinogalactans, likely anchored to type I rhamnogalacturonans (RG-I). Glycosidic linkage studies are needed to confirm the arabinogalactan type. The polysaccharides isolated from umari fruit pulp may exhibit biological activities – such as antitumor, anti-inflammatory, anti-ulcer, antinociceptive effects – similar to those previously reported for arabinogalactans, which should be further investigated. The authors would like to thank CAPES-PROEX and CNPq for their financial support. Author GOMES, D.M.D. received a scholarship from CAPES.