THREE-DIMENSIONAL CULTURED DISSOCIATED MESENCHYMAL STROMAL CELLS MITIGATE IN VITRO NEUTROPHIL EXTRACELLULAR TRAPS INDUCED BY LIPOPOLYSACCHARIDE

Introduction: Neutrophil extracellular traps (NETs) are characterized as extracellular DNA fibers comprised of histone and granular proteins. NETs play a crucial role in defense against systemic infections, inflammation, autoimmune disorders and cancer. However, excessive NETs release can increase inflammatory processes and tissue damage. Mesenchymal stromal cells (MSCs) are a promising therapy to mitigate immune dysfunctions. However, MSCs behavior can be influenced by in vitro conditions. For instance, MSCs cultured in two-dimensional (2D) or three-dimensional (3D) conditions can reduce NETs formation has not been evaluated.